Thiamin-Binding Protein from Sunflower Seeds.
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چکیده
منابع مشابه
Thiamin-binding protein from sunflower seeds.
A thiamin-binding protein was isolated from sunflower seeds. Its molecular mass was estimated to be 230 kDa by gel filtration. The protein was suggested to be composed of six subunits, which consisted of polypeptides linked by disulfide bond(s). The protein contained a large amount of glutamine or glutamic acid (19.9 mol%) and asparagine or aspartic acid (11.1 mol%). The levels of tryptophan an...
متن کاملAccumulation and degradation of thiamin-binding protein and level of thiamin in wheat seeds during seed maturation and germination.
Changes in the levels of thiamin-binding globulin and thiamin in wheat seeds during maturation and germination were studied. The thiamin-binding activity of the seed proteins increased with seed development after flowering. The thiamin content of the seeds also increased with development. Thiamin-binding activity decreased during seed germination. On the other hand, immunological analysis using...
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This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity.
متن کاملOxidative phosphorylation by mitochondria extracted from dry sunflower seeds.
The role of mitochondria in the phosphorylation of ADP to ATP in the early steps of seed germination has been studied. Mitochondria were extracted from dry sunflower (Helianthus annuus) seeds. Adenylate kinase-dependent ATP synthesis was inhibited by p(1),p(5)-di(adenosine-5')pentaphosphate. Synthesis of ATP was observed with the different substrates: citrate, alpha-ketoglutarate, succinate, ma...
متن کاملUnusual cause of constipation: sunflower seeds bezoar.
Bezoars are accumulated foreign material that forms concretions in the gastrointestinal tract, inducing abdominal pain, constipation, obstruction, and even peritonitis. This case report describes a rare rectal bezoar in a healthy young woman who had recently consumed large quantities of sunflower seeds. A 21-year-old healthy woman was admitted with abdominal pain, rectal pain, and constipation ...
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ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 1998
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.44.665